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聯系人：楊燕燕
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技術文章
WHSC2 [H8-B11] Catalog Number：OM627602
Product Profile
| Product Name | WHSC2 [H8-B11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 More ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Concentration | 2 mg/mL. |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Nucleus. |
Database Links
| SwissProt ID | Q9H3P2 Q5U2N1 |
|---|
Application
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Application
Fig1: Western blot analysis of WHSC2 on human WHSC2 recombinant protein using anti-WHSC2 antibody at 1/1,000 dilution.
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Application
Fig2: Western blot analysis of WHSC2 on HEK293 (1) ａnd WHSC2-hIgGFc transfected HEK293 (2) cell lysate using anti-WHSC2 antibody at 1/1,000 dilution.
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Application
Fig3: Western blot analysis of WHSC2 on different cell lysate using anti-WHSC2 antibody at 1/1,000 dilution. Positive control： Line1:Jurkat Line2:HeLa Line3:HEK293 Line4:Rat brain Line5:A549 Line6:SPC-A-1
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Application
Fig4: ICC staining WHSC2 (green) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.
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Application
Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.
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Application
Fig7: Flow cytometric analysis of HEK293 cells with WHSC2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).
| Positive Control | Human WHSC2 recombinant protein, WHSC2-hIgGFc transfected HEK293 cell lysate, Jurkat, HeLa, HEK293, A549, SPC-A-1 cell lysate, Rat brain tissue lysate, HeLa, human colon cancer tissue, human ovarian cancer tissue, HEK293. |
|---|---|
| Application Notes | WB:1:250~1:1000 More ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
原創作者：上海篤瑪生物科技有限公司
