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技術文章
NLRP3 [SC06-23] Catalog Number：OM627352
Product Profile
| Product Name | NLRP3 [SC06-23] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | recombinant protein |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000 More ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1 mg/mL. |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 118/85 kDa |
| Cellular Localization | Cylasm, Inflammasome, Secreted, Nucleus, Endoplasmic reticulum. |
Database Links
| SwissProt ID | Q96P20 Q8R4B8 287362 |
|---|
Application
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Application
Fig1: Western blot analysis of NLRP3 on human lung tissue lysates. Proteins were transferred to a PVDF membrane ａnd blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Application
Fig2: ICC staining NLRP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig3: ICC staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig4: ICC staining NLRP3 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.
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Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.
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Application
Fig7: Flow cytometric analysis of Jurkat cells with NLRP3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
| Positive Control | Human lung tissue lysates, Hela, HUVEC, PMVEC, mouse bladder tissue, mouse spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000 More ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
原創作者：上海篤瑪生物科技有限公司
