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聯系人：楊燕燕
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技術文章
GRP78 antibody Catalog Number：OM255573
Product Profile
| Product Name | GRP78 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Hspa5. The exact sequence is proprietary. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. : | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1 mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
Target Information
| Gene Synonyms | More |
|---|---|
| Molecular Weight(MW) | 72 kDa (note) (2) |
| Tissue Specificity | Knockdown/Knockout validation was supported by customer review data. |
| Cellular Localization | Endoplasmic reticulum lumen , Melanosome |
Application
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Application
Hspa5 antibody detects Hspa5 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hspa5 protein stained by Hspa5 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
-
Application
Hspa5 antibody detects Hspa5 protein by Western blot analysis. A. 50 µg Rat heart lysate/extract B. 50 µg Rat Lung lysate/extract C. 50 µg Rat Liver lysate/extract D. 50 µg Rat kidney lysate/extract 7.5 % SDS-PAGE Hspa5 antibody dilution: 1:10000
-
Application
Hspa5 antibody detects Hspa5 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Hspa5 antibody dilution: 1:500.
-
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 E: A375 7.5% SDS PAGE diluted at 1:10000
-
Application
Sample (30 ug of whole cell lysate) A: NIH-3T-3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 7.5% SDS PAGE diluted at 1:10000
-
Application
Hspa5 antibody detects Hspa5 protein at cylasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Hspa5 antibody diluted at 1:500.
-
Application
Hspa5 antibody detects Hspa5 protein at cylasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Hspa5 antibody diluted at 1:500.
| Positive Control | 293T , A431 , HeLa , HepG2 , A375 , NIH-3T-3 , JC , BCL-1 , C2C12 , Raw264.7 , rat heart , rat lung , rat liver , rat kidney |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. : |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 0.05% Sodium Azide was added as a preservative. |
原創作者：上海篤瑪生物科技有限公司
