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              技術文章

              CD33 Catalog Number:OM640668

              點擊次數:25 發布時間:2024/8/29 14:36:21
               

              Product Profile

              Product Name CD33
              Antibody Type Primary Antibodies
              Immunogen
              Purified recombinant fragment of human CD33 (AA: 15-237) expressed in E. Coli.

              Key Feature

              Clonality Monoclonal
              Host Species Unknow
              Tested Applications

              WBIHCFCMICC/IF

               
              WB:1:250~1:1000
              IHC:1:20~1:100
              ICC/IF:1:100~1:200
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              More 
              Species Reactivity

              Human

              Target Information

              Alternative Names
              p67
              SIGLEC3
              SIGLEC-3
              Molecular Weight(MW) 39.8kDa

              Database Links

              Entrez Gene 945

              Application

              • Application

                Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

              • Application

                Figure 1: Western blot analysis using CD33 mAb against human CD33 recombinant protein. (Expected MW is 49.2 kDa)

              • Application

                Figure 2: Western blot analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.

              • Application

                Figure 3: Western blot analysis using CD33 mouse mAb against THP-1 (1) cell lysate.

              • Application

                Figure 5: Flow cytometric analysis of HeLa cells using CD33 mouse mAb (green) and negative control (purple).

              • Application

                Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD33 mouse mAb with DAB staining.

              • Application

                Figure 7: Immunohistochemical analysis of paraffin-embedded endometrium tissues using CD33 mouse mAb with DAB staining.

              Application Notes
              WB:1:250~1:1000
              IHC:1:20~1:100
              ICC/IF:1:100~1:200
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              More 

              Additional Information

              Form Ascitic fluid containing 0.03% sodium azide.
              Storage Instructions 4℃; -20℃ for long term storage

              原創作者:上海篤瑪生物科技有限公司

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