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              技術文章

              CD33 [B7-C6] Catalog Number:OM627666

              點擊次數:28 發布時間:2024/8/29 14:27:43
               

              Product Profile

              Product Name CD33 [B7-C6]
              Antibody Type Primary Antibodies
              Product description
              CD33 is a type I transmembrane glycoprotein that is found on granulocyte and macrophage precursors in the bone marrow, but is absent from pluripotent stem cells. CD33 is also expressed on monocytes in peripheral blood. It is used as a marker to distinguish myelogenous leukemia cells from lymphoid or erythroid leukemias. CD33 may function as a sialic acid-dependent cell adhesion molecule.
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              Immunogen
              Recombinant protein

              Key Feature

              Clonality Monoclonal
              Isotype IgG1
              Host Species Mouse
              Tested Applications

              WBIHCFC

               
              WB:1:250~1:1000
              IHC:1:20~1:100
              FC:1:100~1:200
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
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              Species Reactivity

              Human

              Concentration 2 mg/mL.

              Target Information

              Alternative Names
              CD 33 antibody CD33 antibody CD33 antigen (gp67) antibody CD33 antigen antibody CD33 molecule antibody CD33_HUMAN antibody FLJ00391 antibody gp67 antibody My9 antibody Myeloid cell surface antigen CD33 antibody Myeloid cell surface antigen CD33 precursor antibody Myeloid differentiation antigen CD33 antibody p67 antibody Sialic acid binding Ig like lectin 3 antibody Sialic acid binding immunoglobulin like lectin 3 antibody Sialic acid-binding Ig-like lectin 3 antibody SIGLEC 3 antibody Siglec-3 antibody SIGLEC3 antibody
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              Molecular Weight(MW) 70 kDa
              Cellular Localization Cell membrane.

              Database Links

              SwissProt ID P20138

              Application

              • Application

                Fig1: Western blot analysis of CD33 on human CD33 recombinant protein using anti-CD33 antibody at 1/1,000 dilution.

              • Application

                Fig2: Western blot analysis of CD33 on HEK293 (1) and CD33-hIgGFc transfected HEK293 (2) cell lysate using anti-CD33 antibody at 1/1,000 dilution.

              • Application

                Fig3: Western blot analysis of CD33 on THP-1 (1)  cell lysate using anti-CD33 antibody at 1/1,000 dilution.

              • Application

                Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CD33 antibody. Counter stained with hematoxylin.

              • Application

                Fig5: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-CD33 antibody. Counter stained with hematoxylin.

              • Application

                Fig6: Flow cytometric analysis of Hela cells with CD33 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

              Positive Control Human CD33 recombinant protein, CD33-hIgGFc transfected HEK293 cell lysate, THP-1 cell lysate, human esophageal cancer tissue, human endometrium tissue, Hela.
              Application Notes
              WB:1:250~1:1000
              IHC:1:20~1:100
              FC:1:100~1:200
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              More 

              Additional Information

              Form Liquid
              Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
              Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

              原創作者:上海篤瑪生物科技有限公司

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