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聯系人：楊燕燕
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技術文章
CD33 Catalog Number：OM629680
Product Profile
| Product Name | CD33 |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | KLH conjugated synthetic peptide derived from mouse CD33 101-200/334 |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1 mg/mL. |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 40/37 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. |
Database Links
| SwissProt ID | Q63994 |
|---|
Application
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Application
Fig1: Blank control: mouse spleen cells (blue). Primary Antibody: Rabbit Anti- CD33 antibody(ER1905-89), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1905-89, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
-
Application
Fig2: Sample: A549 Cell (Human) Lysate at 30 ug Primary: Anti-CD33 (ER1905-89) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/37 kD Observed band size: 40 kD
| Positive Control | Monocytic/myeloid lineage cells. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol. |
原創作者：上海篤瑪生物科技有限公司
