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              技術文章

              CD33 Antibody Catalog Number:OM274456

              點擊次數:23 發布時間:2024/8/29 14:21:12
               

              Product Profile

              Product Name CD33 Antibody
              Antibody Type Primary Antibodies
              Product description
              CD33 Antibody: CD33 is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family that is highly expressed on myeloid progenitor cells. Assessment of CD33 expression is of great importance in the immunodiagnosis of acute leukemia, allowing distinction between myeloid and lymphoid origin, as CD33 is generally restricted to the myelomonocytic lineage. CD33 can associate with the protein-tyrosine phosphatases SHP-1 and SHP-2 and thus could modulate downstream signaling events associated with cell activation. Common variants of CD33 have been found to be associated with late-onset Alzheimer's disease.1) Freeman SD, Kelm S, Barber EK, et al. Characterization of CD33 as a new member of the sialoadhesion family of cellular interaction molecules. Blood 1995; 85:2005-12.
              More 
              Immunogen
              CD33 antibody was raised against a 14 amino acid synthetic peptide near the center of human CD33. The immunogen is located within amino acids 180 - 230 of CD33.

              Key Feature

              Clonality Polyclonal
              Isotype IgG
              Host Species Rabbit
              Tested Applications

              ELISAWB

               
              CD33 antibody can be used for detection of CD33 by Western blot at 1 - 2 μg/mL.:
              Species Reactivity

              HumanMouseRat

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Symbol CD33
              Alternative Names
              CD33 Antibody: p67
              SIGLEC3
              SIGLEC-3
              Myeloid cell surface antigen CD33
              Sialic acid-binding Ig-like lectin 3
              Siglec-3
              More 
              Tissue Specificity At least three isoforms of CD33 are known to exist; this antibody will detect all three isoforms.

              Database Links

              Entrez Gene 945
              Protein Accession NP_001763

              Application

              • Application

                Western blot analysis of CD33 in 3T3 cell lysate with CD33 antibody at (A) 1 and (B) 2 μg/mL.

              Positive Control 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
              Application Notes
              CD33 antibody can be used for detection of CD33 by Western blot at 1 - 2 μg/mL.:

              Additional Information

              Form Liquid
              Storage Instructions CD33 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer CD33 Antibody is supplied in PBS containing 0.02% sodium azide.
               

              原創作者:上海篤瑪生物科技有限公司

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