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              技術文章

              FLT3 antibody Catalog Number:OM253326

              點擊次數:20 發布時間:2024/8/26 13:43:33
               

              Product Profile

              Product Name FLT3 antibody
              Antibody Type Primary Antibodies
              Product description
              Rabbit Polyclonal antibody to FLT3FLT3 encodes a class III receptor tyrosine kinase that regulates hemaoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cylasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to auhosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cylasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hemaoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
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              Immunogen
              Peptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3.

              Key Feature

              Clonality Polyclonal
              Host Species Rabbit
              Tested Applications

              ICC/IFWB

               
              Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
              Species Reactivity

              Human

              Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
              Purification Affinity purified

              Target Information

              Gene Synonyms
              CD135
              FLK2
              STK1
              FLT3
              FLT3 receptor tyrosine kinase
              STK-1
              FLT-3
              CD135 antigen
              fetal liver kinase-2
              stem cell tyrosine kinase 1
              tyrosine-protein kinase receptor FLT3
              growth factor receptor tyrosine kinase type III
              fms-like tyrosine kinase 3
              FL c
              More 
              Target FLT3
              Molecular Weight(MW) 130, 160 (note)

              Application

              • Application

                Western blot analysis of extracts from 293 and MCF cells using FLT3 Antibody .

              • Application

                Immunofluorescence staining of methanol-fixed Hela cells using FLT3 Antibody .

              Application Notes
              Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

              Additional Information

              Form Liquid
              Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
              Storage Buffer saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

              原創作者:上海篤瑪生物科技有限公司

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