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              技術文章

              FLT3 Antibody Catalog Number:OM278969

              點擊次數:19 發布時間:2024/8/26 13:41:27
               

              Product Profile

              Product Name FLT3 Antibody
              Antibody Type Primary Antibodies
              Product description
              FLT3 encodes a class III receptor tyrosine kinase that regulates hemaoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cylasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to auhosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cylasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hemaoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.1) Sekine S et, al. (2008) J Immunol. ;180 (12): 8126-34Pratz K et, al. (2008) Leuk Lymphoma. 2008 ;49 (5): 852-63. Al Shaer L et, al. (2008) Br J Haematol. 141 (4): 483-93.
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              Immunogen
              FLT3 (Ab-591) antibody was raised against a peptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3.

              Key Feature

              Clonality Polyclonal
              Host Species Rabbit
              Tested Applications

              IFWB

               
              Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200:
              Species Reactivity

              Human

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Symbol FLT3
              Alternative Names
              FLK2
              STK1
              CD135
              FLK-2
              CDKN6
              FLT-3
              FLT3
              STK-1
              More 
              Molecular Weight(MW) 130, 160 kDa
              Tissue Specificity This antibody detects endogenous level of total FLT3 protein.

              Database Links

              Entrez Gene 2322
              Protein Accession NP_004110.2

              Application

              • Application

                Immunofluorescence staining of methanol-fixed HeLa cells using FLT3 (Ab-591).

              • Application

                Western blot analysis of lysed extracts from 293 and MCF cells using FLT3 (Ab-591).

              Application Notes
              Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200:

              Additional Information

              Form Liquid
              Storage Instructions Store antibody at -20˚C for up to one year.
              Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

              原創作者:上海篤瑪生物科技有限公司

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