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技術文章
查找：Transcreener ADP2 TR-FRET Red Assay
一般描述
The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, ａnd greatly simplifies compound ａnd inhibitor profiling across multiple target families.
The Transcreener® ADP2 FP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of ADP ａnd therefore is compatible with any enzyme class that produces ADP, including protein, lipid, ａnd carbohydrate kinases, ATPases, DNA helicases, carboxylases ａnd glutamine synthetase. The Transcreener® ADP2 Assay is a simple one step homogenous detection assay, ａnd is extremely flexible with regard to ATP concentration (0.1 to 1,000 μM ATP). The assay provides excellent signal at low substrate conversion, with a Z′ = 0.7 ａnd = 85 polarization shift (mP) at 10% ATP conversion using 1 μM ATP.
The Transcreener® ADP2 FP Assay was developed to follow the progress of any enzyme that produces ADP. The Transcreener® ADP Detection Mixture comprises an ADP Alexa633 Tracer bound to an ADP2 Antibody. The tracer is displaced by ADP, the invariant product generated during the enzyme reaction.The displaced tracer freely rotates leading to a decrease in fluorescence polarization. The assay uses a far red tracer to minimize interference from fluorescent compounds ａnd light scattering.
View full Transcreener® product list
數量
3010-1K = 1,000 assay, 384-well
3010-10K = 10,000 assay, 384-well
外形
Kit with buffered aqueous solutions
法律信息
Transcreener is a registered trademark of BellBrook Labs
。
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上海篤瑪生物科技有限公司經過數年的努力發展已迅速成為集產品研發、生產、經營為一體的業化生物工程公司。公司具有完善的實驗技術開發平臺，成熟的抗原、抗體研發系統，熟練掌握各種酶聯技術，結合公司的研發團隊，可以有效的保證公司產品強的穩定性和高的準確性，同時又具有競爭力的價格。
公司目主要提供生化試劑、分子生物學試劑及試劑盒，各種抗體及免疫學試劑盒、實驗耗材等多門類上萬種產品，產品涉及分子生物學、細胞生物學、免疫學等生命科學的各個域。
原創作者：上海篤瑪生物科技有限公司
