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技術文章
推薦：Type I Collagen Solution, 3 mg/ml (Human)
Catalog #5007
VitroCol® is a 3 mg/ml, type I human atelocollagen solution for 2D coatings, 3D hydrogels, or as a collagen standard. VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells, then processed ａnd purified to yield the naturally produced human collagen.Product DescriptionVitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity ( 99% collagen content), functionality ａnd represents the only native-like human collagen offered.
VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblast to naturally ａnd efficiently secret extracellular matrix. The extracellular matrix is then processed ａnd purified to yield the naturally produced human collagen.
VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. VitroCol® is supplied at approximately 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. VitroCol® is soluble atelocollagen in 0.01 N HCI, therefore, the pH is 2.
VitroCol® is especially ideal for human cell culture systems when coating of surfaces, providing preparations of thin layers of culturing cells, or use as a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use ａnd storage. VitroCol® is sterile filtered ａnd is supplied as a ready to use solution.
Parameter, Testing, ａnd Method
VitroCol® Type I Collagen #5007
推薦：Type I Collagen Solution, 3 mg/ml (Human)
Sterilization Method
Filtration
Extraction Method
Enzyme - atelocollagen
Form
Solution
Package Size
20 mL, 100 mL
Storage Temperature
2-10°C
Shelf Life
Minimum of 6 months from date of receipt
Collagen Concentration - Biuret
2.9-3.2 mg/mL
Collagen Purity - Silver Staining
99%
pH
1.9-2.1
Kinetic Gel Test (Minutes)
40
Gel Formation Tube Test (Minutes)
40
Fibrillogenesis (Absorbance Units)
0.5
Electrophoretic Pattern - Coomassie Blue
Characteristic
Sterility - USP modified
No growth
Endotoxin - LAL
5.0 EU/mL
Osmolality (mOsmo H2O/kg)
35
Cell Attachment Assay
Pass
Source
Human Neo-Natal Fibroblasts
Hydrogel Young s Modulus E (Pa)
Characteristic
Product ReferencesReferences for VitroCol®:
Shieh, Hester F., et al. Comparisons of human amniotic mesenchymal stem cell viability in FDA-approved collagen-based scaffolds: Implications for engineered diaphragmatic replacement. Journal of pediatric surgery 52.6 (2017): 1010-1013.
van der Velden, Jos LJ, et al. Transforming Growth Factor-? Induces A Mesenchymal Profile In Human Nasal Epithelial Cells. D76. ALVEOLAR EPITHELIUM: NOVEL TOOLS AND PHENOTYPES. American Thoracic Society, 2012. A6322-A6322.
Tashima, Takumi, et al. Osteomodulin regulates diameter ａnd alters shape of collagen fibrils. Biochemical ａnd biophysical research communications 463.3 (2015): 292-296.
Spiller, Kara L., Suzanne A. Maher, ａnd Anthony M. Lowman. Hydrogels for the repair of articular cartilage defects. Tissue engineering part B: reviews 17.4 (2011): 281-299.
Brilha, Sara, et al. Monocyte adhesion, migration, ａnd extracellular matrix breakdown are regulated by integrin αVβ3 in Mycobacterium tuberculosis infection. The Journal of Immunology 199.3 (2017): 982-991.
Jonsdottir, Hulda R., ａnd Ronald Dijkman. Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures. Coronaviruses. Humana Press, New York, NY, 2015. 73-87.
Sabbione, Florencia, et al. Neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells. Journal of innate immunity 9.4 (2017): 387-402.
Dos Santos Brilha, S., et al. Monocyte adhesion, migration ａnd extracellular matrix breakdown is regulated by integrin αVβ3 in Mycobacterium tuberculosis infection.
Brilha, Sara, et al. Monocyte Adhesion, Migration, ａnd Extracellular Matrix Breakdown Is Regulated by Integrin aVb3 in Mycobacterium tuberculosis Infection. (2017).
Colace, T., et al. Analysis of morphology of platelet aggregates formed on collagen under laminar blood flow. Annals of biomedical engineering 39.2 (2011): 922-929.
Muthard, Ryan W., ａnd Scott L. Diamond. Blood clots are rapidly assembled hemodynamic sensors: flow arrest triggers intraluminal thrombus contraction. Arteriosclerosis, thrombosis, ａnd vascular biology 32.12 (2012): 2938-2945.
Maloney, S. F., Lawrence F. Brass, ａnd S. L. Diamond. P2Y12 or P2Y1inhibitors reduce platelet deposition in a microfluidic model of thrombosis while apyrase lacks efficacy under flow conditions. Integrative Biology 2.4 (2010): 183-192.
Bauer, Rebecca N., et al. Interaction with epithelial cells modifies airway macrophage response to ozone. American journal of respiratory cell ａnd molecular biology 52.3 (2015): 285-294.
Kawamura, Shunsuke, et al. Identification of common monocyte progenitors, pre-monocytes, ａnd granulocyte monocyte progenitors in human umbilical cord blood. Experimental Hematology 43.9 (2015): S72.
IF<5，贈送價值 500元禮品卡
5 ≤IF≤10，贈送價值 1000元禮品卡
IF≥10，贈送價值 1000元禮品卡+任意 1 款 ValukineTM 試劑盒
DUMABIO ELISA試劑盒文獻征集獎勵活動
為感謝各位老師對篤瑪品牌的支持與厚愛，凡購買DUMABIO ELISA試劑盒，在SCI期刊雜志發表文中注明ELISA試劑盒采購上海篤瑪生物科技有限公司（Shanghai DuMa Biotechnology Co., Ltd.）
Shanghai DuMa Biotechnology Co., Ltd.
發表刊物 影響因子 獎勵金額
SCI期刊雜志 1.0≤1F＜3.0 200元
SCI期刊雜志 3.0≤1F＜6.0 400元
SCI期刊雜志 6.0≤1F＜10.0 600元
SCI期刊雜志 1F≥10 1000元
原創作者：上海篤瑪生物科技有限公司
