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技術文章
Anti-puromycin [3RH11]
| Product Type: | Antibody |
| Name: | Anti-Puromycin (3RH11) |
| Host: | Mouse |
| Isotype: | IgG1 kappa |
| Clonality: | Monoclonal |
| Clone Name: | 3RH11 |
| Specificity: | This antibody recognizes puromycin. |
| Immunogen: | puromycin hydrochloride |
| Format: | Liquid |
| Purity: | Protein G purified |
| Buffer: | PBS with 0.02% Proclin 300 |
| Tested Applications: | Western blotting (1:1,000), ELISA ａnd Immunofluorescence microscopy. |
| Storage: | +4C (short-term), -20C (long-term); Avoid repeated freeze/thaw cycles. |
| Shipped: | Cold packs |
Anti-puromycin [3RH11]
Description
EQ0001，This monoclonal antibody to puromycin provides a non-radioactive method to measure rates of global protein synthesis (mRNA translation) in cells or tissue slices incubated with puromycin, or animals treated with puromycin in vivo.
Highlights:
* Allows for the simple evaluation ａnd quantification of translation directly using standard immunochemical methods
* Advantageous alternative to traditional pulse-chase methods, which rely on radioactive amino acid labeling
* Compatible with Western Blot ａnd ELISA applications
* Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma 3RH11
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. Part of the molecule resembles the 3＇ end of the aminoacylated tRNA, making it useful for protein translation analysis. Classical pulse-chase or flooding dose methods used to monitor protein synthesis rely on the measurement of radioactive methionine ａnd cysteine labels. Analysis using puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling, ａnd allows for the evaluation/quantification of translation directly using standard immunochemical methods
科研ELISA是上海篤瑪生物的主營產品，科研ELISA欄目提供該類目產品說明書以及提供產品報價與網上訂購。
上海篤瑪生物科技有限公司經過數年的努力發展已迅速成為集產品研發、生產、經營為一體的業化生物工程公司。公司具有完善的實驗技術開發平臺，成熟的抗原、抗體研發系統，熟練掌握各種酶聯技術，結合公司的研發團隊，可以有效的保證公司產品強的穩定性和高的準確性，同時又具有競爭力的價格。
公司目主要提供生化試劑、分子生物學試劑及試劑盒，各種抗體及免疫學試劑盒、實驗耗材等多門類上萬種產品，產品涉及分子生物學、細胞生物學、免疫學等生命科學的各個域。
原創作者：上海篤瑪生物科技有限公司
