Biotium的Steady-Luc™HTS檢測系統是一種有的混合物，可通過防止在酶表面形成腺苷酰氧化熒光素來改變酶促反應，從而產生持久的信號（穩定發光）。 它是一種同質的高靈敏度螢火蟲熒光素酶報告基因測定試劑盒，具有約3小時的半衰期，用于定量哺乳動物細胞中的螢火蟲熒光素酶表達，允許在微孔板中進行批量處理。與閃光型檢測相比，發光型熒光素酶檢測（如Steady-Luc™）的發光信號較低。 檢測的靈敏度和限將取決于實驗系統中的熒光素酶表達水平以及光度計靈敏度。

Biotium還提供Steady-Luc™Firefly HTS檢測試劑盒（凍干），凍干檢測緩沖液便于室溫運輸和-20°C儲存。

艾美捷Firefly HTS檢測試劑盒#BTM-30028-1是一種均相、高靈敏度的螢火蟲熒光素酶報告基因檢測試劑盒。它是一種發光型檢測，信號半衰期約為 3 小時，可在微孔板中進行批處理。

組件：

D-熒光素

Steady-Luc™ 螢火蟲檢測緩沖液

特征：

簡單的單步均相測定

發光半衰期約3小時

物超所值：與其他品牌相比節省高達45%

Firefly HTS檢測試劑盒屬性：

化驗類型/選項：高通量分析、均質分析、發光（發光型）

檢測方法/讀數：微孔板讀取器（發光）

儲存條件：在-60°C或更低溫度下儲存

Firefly Luciferase is widely used as a reporter gene to study gene regulation ａnd function ａnd drug screening. Due to the lack of endogenous activity in mammalian cells or tissues, it is a highly sensitive genetic reporter gene. However, the light generated by the reaction leads to the formation of suicidal adenosyloxyfluorescein on the surface of the enzyme. It results in a very short half-life of light emission with flashing dynamics. Because of this, flash type luminescence must be detected immediately after the reaction begins, which is incompatible with batch analysis of samples.

Steady Luc from Biotium ™  The Firefly HTS detection kit contains a proprietary mixture of substances that can prevent adenosine oxyfluorescein reactions ａnd generate persistent luminescent signals. It generates a luminescent signal with a half-life of approximately 3 hours for high-throughput microplate detection.

Compared to flash type determination, Steady Luc ™  Isoluminescent Luciferase assay has low luminescence signal. The sensitivity ａnd detection limit of this determination will depend on the expression level of Luciferase in your experimental system ａnd the sensitivity of the photometer.

來源：https://www.amyjet.com/products/BTM-30028-1.shtml