艾美捷海腎熒光素酶測定試劑盒2.0,150次測定#BTM-30082-1組件：

5X 被動裂解緩沖液

海腎螢光素酶檢測緩沖液 2.0

Aquaphile™ 腔腸素

海腎熒光素酶測定試劑盒相關特征：

高靈敏度和線性快速型 RLuc 測定

易于處理的水溶性基材

高性價比

海腎熒光素酶測定試劑盒屬性：

化驗類型/選項：終點測定，發光（閃光型）

檢測方法/讀數：光度計（帶試劑注射器的單管或微量板讀取器）

儲存條件：在-60°C或更低溫度下儲存

Sea kidney Luciferase has been used as a reporter gene to study gene regulation ａnd function in vitro ａnd in vivo. It is usually used for multiple transcriptional reporter gene detection or as a standardized transfection control for firefly Luciferase detection. Renila Luciferase is a 36000 Dalton monomer protein that catalyzes the oxidation of coelenterazine by oxygen to produce light. The activity of this enzyme does not require post translational modification ａnd can serve as a genetic reporter gene immediately after translation. Coelenterazine also emits light through enzyme independent oxidation, a process called self luminescence. Superoxide anions ａnd peroxynitrite in cells ａnd tissues enhance self luminescence. This test kit uses a special buffer formula, which aims to conduct reliable linear measurement of Luciferase activity in the sea ａnd kidney with the minimum self luminous background ａnd excellent sensitivity.

Note: This reagent kit is a flash type luminescence detection that requires immediate measurement of luminescence after adding reagents to your sample. It requires a single tube luminescent meter or a luminescent enzyme-linked immunosorbent assay instrument with a reagent syringe.

Easy to use for consistent results

This kit is a new improved substitute for Haishen Luciferase Test Kit (Catalog No. 30004). In addition to providing a simplified experimental scheme, Renilla Luciferase Test Kit 2.0 also has Biotium Aquaphile ™  Coelenterazine, a water-soluble substrate, is different from coelenterazine＇s methanol solution. It can be stored at - 20 ° C with the minimum evaporation.

The measurement quantity is based on a 96 well measurement format. This kit contains sufficient passive lysis buffer to perform the specified number of measurements within the plate size range of 96 to 24 wells.

來源：https://www.amyjet.com/products/BTM-30082-1.shtml