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親和純化的抗黑色素蛋白(UF008)是針對小鼠黑色素蛋白酶的15個N端胞外氨基酸而產生的。這種抗體不會與其他物種的黑素蛋白發生交叉反應,它對小鼠非常特異。
Advanced Targeting Systems/ATSbio艾美捷兔黑色素蛋白多克隆,親和純化抗體:
圖:使用AB-N39在1:4000的平板小鼠視網膜和AlexaFluor-568二抗體中從單個z系列獲得的2-光子共焦顯微鏡圖像。這張圖像是通過神經節細胞層的單個視平面,也顯示了一些近端RGC樹突。圖片由Matthew Van Hook博士提供。
Advanced Targeting Systems/ATSbio艾美捷兔黑色素蛋白多克隆,親和純化抗體應用包括免疫熒光(1µg/ml)和靶向(黑色素蛋白SAP中的靶向劑,目錄號IT-44)。這種抗體在橫截面染色和平板染色中都有效。
Advanced Targeting Systems/ATSbio 艾美捷其它熱門抗體研究:
AB-N39 Melanopsin Rabbit Polyclonal, affinity-purified 黑視素(Melanopsin)
AB-N01AP NGFr (mu p75) Rabbit Polyclonal, affinity-purified NGFr (mu p75)
AB-N07 NGFr (ME20.4, p75) Mouse Monoclonal NGFr (ME20.4, p75)
AB-N33AP NK-1 Receptor Rabbit Polyclonal, affinity-purified NK-1受體
AB-N03 trkA Rabbit Polyclonal trkA
Advanced Targeting Systems/ATSbio艾美捷兔黑色素蛋白多克隆抗體相關文獻:
Examination of zinc in the circadian system.
Moshirpour M, Nakashima AS, Sehn N, Smith VM, Thackray SE, Dyck RH, Antle MC (2020) Examination of zinc in the circadian system. Neuroscience 432:15-29. doi: 10.1016/j.neuroscience.2020.02.016
Objective: To examine the anatomical and functional aspects of zinc in the circadian system.
Summary: Neither enhancement nor chelation of free zinc at either the SCN or IGL altered circadian responses to phase-shifting light in hamsters.
Usage: Retinal immunohistochemistry (1:5000) included a 20-min wash in 4% PFA prior to initiation of the IHC protocol.
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
nGnG amacrine cells and Brn3b-negative M1 ipRGCs are specifically labeled in the ChAT-ChR2-EYFP mouse.
Cui LJ, Chen WH, Liu AL, Han X, Jiang SX, Yuan F, Zhong YM, Yang XL, Weng SJ (2020) nGnG amacrine cells and Brn3b-negative M1 ipRGCs are specifically labeled in the ChAT-ChR2-EYFP mouse. Invest Ophthalmol Vis Sci 61(2):14. doi: 10.1167/iovs.61.2.14
Summary: The authors speculated that type II cells might be ipRGCs. This was later verified by the strong immunostaining of type II cells in response to the melanopsin antibody UF006 (100%, 141 of 141 cells collected from 5 retinas, Figs. 6B1–B3), which probes multiple ipRGC subtypes.
Usage: Immunostaining 1:10000
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
Advanced Targeting Systems(ATSbio)主要研究域包含神經靶向毒素,神經生物學研究方面的抗體/蛋白,靶向劑內化水平檢測工具等。艾美捷科技是ATSbio的中國代理商,為科研工作者提供優質的產品與服務。
來源:https://www.amyjet.com/brand/Advanced-Targeting-Systems.shtml